rep-PCR (Repetitive Sequence-Based PCR) #
Prokaryotic and eukaryotic genomes contain dispersed repetitive sequences separating longer single-copy DNA sequences. Interspersed repetitive sequences are characterized as relatively short (usually <500 bp), non-coding, dispersed elements in bacterial genomes. Current data supports that repetitive DNA comprises a substantial portion of the microbial genomes. rep-PCR primers complement these repetitive sequences and allow for specific binding providing reproducible, unique rep-PCR DNA fingerprint patterns.
Generation of Organism Specific rep-PCR DNA Fingerprint Patterns
rep-PCR의 원리 #
Rep-PCR primers bind to many specific repetitive sequences interspersed throughout the genome.
Multiple Fragments of various lengths are amplified
Fragments can be separated by mass and charge via electrophoresis
A unique rep-PCR DNA fingerprint profile created with multiple bands of varying intensity