rep-PCR
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ykkang@insilicogen.com
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rep-PCR (Repetitive Sequence-Based PCR) #
Prokaryotic and eukaryotic genomes contain dispersed repetitive sequences separating longer single-copy DNA sequences. Interspersed repetitive sequences are characterized as relatively short (usually <500 bp), non-coding, dispersed elements in bacterial genomes. Current data supports that repetitive DNA comprises a substantial portion of the microbial genomes. rep-PCR primers complement these repetitive sequences and allow for specific binding providing reproducible, unique rep-PCR DNA fingerprint patterns.
Generation of Organism Specific rep-PCR DNA Fingerprint Patterns
rep-PCR의 원리 #
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Rep-PCR primers bind to many specific repetitive sequences interspersed throughout the genome.
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Multiple Fragments of various lengths are amplified
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Fragments can be separated by mass and charge via electrophoresis
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A unique rep-PCR DNA fingerprint profile created with multiple bands of varying intensity
