Transrate: #

Software for de-novo transcriptome assembly quality analysis. - Genome Res.2016.

Overview #

• by inspecting the contig sequences
• by mapping reads to the contigs and inspecting the alignments
• by aligning the contigs against proteins or transcripts from a related species and inspecting

command-line interface #

Transrate v1.0.3 by Richard Smith-Unna, Chris Boursnell, Rob Patro, Julian Hibberd, and Steve Kelly

DESCRIPTION: Analyze a de-novo transcriptome assembly using three kinds of metrics:

1. sequence based (if --assembly is given)
2. read mapping based (if --left and --right are given)
3. reference based (if --reference is given)

Documentation at http://hibberdlab.com/transrate

• USAGE: transrate

OPTIONS: #

• --assembly: Assembly file(s) in FASTA format, comma-separated
• --left: Left reads file(s) in FASTQ format, comma-separated
• --right: Right reads file(s) in FASTQ format, comma-separated
• --reference: Reference proteome or transcriptome file in FASTA format
• --threads: Number of threads to use (default: 8)
• --merge-assemblies: Merge best contigs from multiple assemblies into file
• --output: Directory where results are output (will be created) (default: transrate_results)
• --loglevel: Log level. One of [error, info, warn, debug] (default: info)
• --install-deps: Install any missing dependencies. One of [read, ref, all]
• --examples: Show some example commands with explanations