International Kockout Mouse Consortium(IKMC) #

개요 #

2007년 3월, Brussels 회의에서 발족한 범 국가적인 프로젝트로써 마우스 유전체 내의 모든 단백질 코딩유전자 각각에 대한 유전자결손마우스배아줄기세포주(mouse embryonic stem cell line)제작, 저장 및 공유활동을 한다. 미국 NIH를 중심으로 유럽, 케나다의 기관들이 참여하였으며, 2012년까지 전체 유전자의 95%에 대한 배아줄기 세포주 보유를 목적으로 하였다. 2011년 약 17000개의 유전자에 대한 conditional knockout 및 complete knockout 마우스 배아줄기세포를 제작하여 보유하고 있다.

목적 #

1. C56BL/6 근교계 마우스의 유전체 내의 ~23,000개 단백질 코딩유전자 각각에대한 유전자결손마우스배아줄기세포주 제작
2. 제작된 배아줄기세포주의 저장
3. 유전자결손마우스배아줄기세포주 제작 방법의 표준화

IKMC allele types #

All IKMC allele type #

관련기관 #

1. European Conditional Mouse Mutagenesis Programme(EUCOMM)

   • German Research Center for Environmental Health GmbH(GSF), Germany
   • University of Frankfurt, Germany
   • Charite, Berlin, Germany
   • University of Technology, Dresden
   • Institut Clinique de la Souris, Strasbourg, France
   • European Molecular Biology Laboratory (EMBL) Monterotondo, Italy
   • Consiglio Nazionale delle Ricerche, Monterotondo, Italy
   • The Wellcome Trust Sanger Institute Hinxton, UK
   • Medical Research Council (MRC), Mammalian Genetics Unit, Harwell

2. North American Conditional Mouse Mutagenesis Programme(NorCOMM)

   • Embryonic Stem Cell/Targeted Mutagenesis Facility, Canada
   • Mammalian Functional Genomics Centre, Canada
   • The Centre for Modeling Human Disease, Canada
   • The Canadian Mouse Mutant Repository, Canada
   • The Hospital for Sick Children, Canada

3. Knockout Mouse Project(KOMP)

   • National Institutes of Health(NIH), US
   • Texas A&M Institute of Genomic Medicine(TIGM), US
   • Children's Hospital Oakland Research Institute(CHORI), US
   • The Wellcome Trust Sanger Institute, UK
   • University of California at Davis, US
   • University of Pennsylvania, US
   • The Jackson Laboratory, US
   • Regeneron Pharmaceuticals, Inc., US

참조 #

1. William C. Skarnes, Barry Rosen, Anthony P. West, Manousos Koutsourakis,Wendy Bushell, Vivek Iyer, Alejandro O. Mujica, Mark Thomas, Jennifer Harrow, Tony Cox, David Jackson, Jessica Severin, Patrick Biggs, Jun Fu,Michael Nefedov, Pieter J. de Jong, A. Francis Stewart & Allan Bradley (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 2011 Jun 15;474(7351):337-42.
2. Ringwald, Martin; Iyer Vivek, Mason Jeremy C, Stone Kevin R, Tadepally Hamsa D, Kadin James A, Bult Carol J, Eppig Janan T, Oakley Darren J, Briois Sebastien, Stupka Elia, Maselli Vincenza, Smedley Damian, Liu Songyan, Hansen Jens, Baldock Richard, Hicks Geoff G, Skarnes William C (Jan 2011). "The IKMC web portal: a central point of entry to data and resources from the International Knockout Mouse Consortium". Nucleic Acids Res. (England) 39 (Database issue): D849–55.
3. Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3.
4. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224.
5. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, et al. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function.". Nature 474 (7351): 337–42.
6. International Mouse Knockout Consortium (2007). "A mouse for all reasons". Cell 128 (1): 9–13.